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Additional file 2: of A chicken bioreactor for efficient production of functional cytokines

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posted on 2018-12-29, 05:00 authored by Lissa Herron, Clare Pridans, Matthew Turnbull, Nikki Smith, Simon Lillico, Adrian Sherman, Hazel Gilhooley, Martin Wear, Dominic Kurian, Grigorios Papadakos, Paul Digard, David Hume, Andrew Gill, Helen Sang
Figure S2. Interferon α2A mono-dispersity, size and purity analysis. A) Size-exclusion chromatography multi-angled light scattering (SEC-MALS) was used to determine the molecular and mono-dispersity of Interferon α2A in solution. Interferon α2A eluted from a Superdex-200 Increase 10/300 GL size exclusion column as a single peak with slight trailing edge (≥ 96% purity; As > 2) with apparent molecular mass of ~ 33.2 ± 2 kDa and an Rs of 2.43 ± 0.2 nm (mean ± SD, n = 3). The molecular mass average across the elution profile is 18.7 kDa with average mono-dispersity (Mw/Mn = 1.038) and no significant aggregation. B) Elution profile of Interferon α2A from an RPC 5 4.6/150 ST column run. Chromatogram shows single sharp peak, with ≥98% purity. (DOCX 486 kb)


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