Additional file 2: Figure S2. of Phosphorylation of tau at Y18, but not tau-fyn binding, is required for tau to modulate NMDA receptor-dependent excitotoxicity in primary neuronal culture

Glutamate-induced neurotoxicity depends on NMDARs and extracellular Ca2+. (A, B) Experiments illustrating Ca2+ dependence of glutamate-induced neurotoxicity (A) and neurotoxicity caused by different glutamate receptor agonists (B). (A) One experiment was performed to confirm that reduction of extracellular Ca2+ counteracts glutamate-induced neurotoxicity. WT neurons were treated with Ca2+-containing isotonic solution or 0 mM Ca2+ solution 1 h prior to and throughout exposure to different concentrations of glutamate. Raw AlamarBlue fluorescence measurements are shown in arbitrary units. (B) Neurotoxicity was assessed as above in WT neuronal cultures treated with the indicated glutamate receptor agonists for 15 min at DIV 13. Data represent means ± SEM of technical replicates (8–16 wells per condition) from 1 to 2 experiments. (PDF 4803 kb)