Additional file 2: Figure S2. of Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application
journal contributionposted on 24.11.2016 by Luciano Guerra, Natalia Faccinetti, Aldana Trabucchi, Bruno Rovitto, Adriana Sabljic, Edgardo Poskus, Ruben Iacono, Silvina Valdez
Any type of content formally published in an academic journal, usually following a peer-review process.
TrxIA-2ic identification by western blot in eluted fractions from size exclusion chromatography and dithiothreitol reduction analysis. WB was revealed with a rabbit polyclonal serum to IA-2ic as primary antibody. A: Identification of monomeric and dimeric forms of TrxIA-2ic. Lane 1: affinity purified TrxIA-2icfrom E. coli GI724 strain; lane 2: eluted fraction at Ve = 8.75 mL, corresponding to dimeric TrxIA-2ic; lane 3: eluted fraction at Ve = 9.79 mL, corresponding to monomeric TrxIA-2ic; B: Conversion to TrxIA-2ic monomeric form under dithiothreitol reduction. Lane 1: TrxIA-2ic under denaturalizing and reducing conditions (8 M urea and 100 mM dithiothreitol). Arrows indicate the electrophoretic mobility of monomeric TrxIA‑2ic. (PDF 186 kb)