Additional file 2: Figure S1. of Integration of quantitated expression estimates from polyA-selected and rRNA-depleted RNA-seq libraries

Variance in expression estimates arising from differential transcriptome sampling by polyA+ and ribo-minus RNA selection methods. Figure S2. Variance in TPM estimates by differential transcriptome sampling was effectively negated by the combined use of a filtered reference transcriptome for quantifying expression, and applying a ratio-based correction to the TPM estimates of ribo-minus libraries. Figure S3. Reduction in the absolute difference in TPM estimates from polyA+ and ribo-minus libraries when applying a ratio-based correction to the latter. Figure S4. Tissue tree constructed from the Euclidean distances between uncorrected TPM vectors for two human RNA-seq datasets sequenced with either polyA+ or ribo-minus libraries. Figure S5. Tissue tree constructed from the Euclidean distances between corrected TPM vectors for two human RNA-seq datasets sequenced with either polyA+ or ribo-minus libraries. Figure S6. Comparison of TPM estimates in the adrenal gland, as generated for 19,716 human genes using both polyA-selected and rRNA-depleted libraries. Figure S7. Comparison of TPM estimates in the liver, as generated for 19,716 human genes using both polyA-selected and rRNA-depleted libraries. Figure S8. Comparison of TPM estimates in the ovary, as generated for 19,716 human genes using both polyA-selected and rRNA-depleted libraries. Figure S9. Comparison of TPM estimates in the sigmoid colon, as generated for 19,716 human genes using both polyA-selected and rRNA-depleted libraries. Figure S10. Comparison of TPM estimates in the spleen, as generated for 19,716 human genes using both polyA-selected and rRNA-depleted libraries. Figure S11. Comparison of TPM estimates in the testis, as generated for 19,716 human genes using both polyA-selected and rRNA-depleted libraries. (DOCX 709 kb)