Additional file 2: Figure S1. of Caprine herpesvirus 2-associated malignant catarrhal fever of captive sika deer (Cervus nippon) in an intensive management system

Pan-herpesvirus detection and DPOL phylogeny of the isolate from affected hinds. Ethidium bromide-stained agarose gel of two amplicons from SP-1 and SP-2 respectively, using the consensus PCR assay targeting herpesviral DNA polymerase (DPOL) (Figure S1A.) Based on the resultant DPOL sequences, a phylogenic tree was constructed using the PhyML software (version 3.0) with LG substitution model (Figure S1B.) Approximate likelihood ratio test (aLRT) was performed and indicated in the node. aLRT values less than 0.50 were collapsed. Scale bar indicates 0.1 amino acid substitutions per site. DPOL GenBank accession number is AAC59454 for outgroup Bovine gammaherpesvirus 4 (BoHV-4); NC_002531 for Alcelaphine herpesvirus 1(AlHV-1); AAO88177 for MCFV-WTD; ADY17131 for Ovine gammaherpesvirus 2 (OvHV-2); APG30119 for Muskox rhadinovirus 1 (Muskox-LHV); ADY17115 for Caprine gammaherpesvirus 2 (CpHV-2); KY475595 for isolate SP-1. (PDF 129 kb)