Additional file 22 of A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells

Additional file 22: Fig. S13. Depletion efficiency and effect on endogenous pst transcript levels. (A,B) The indicated dsRNAs were used for RNAi with SR9rg > pst_E1n (mRuby−, GFP+) cells. Two dsRNA preparations (1 or 2) generated from distinct amplicons were used for some targets. The number of independent experiments is indicated in brackets. In addition, lacZ dsRNA was used in parallel for control. Bars represent average transcript levels as determined by qRT-PCR relative to those detected after lacZ depletion, which were set to 1. Whiskers indicate s.d. in case of multiple independent experiments. (A) To assess RNAi efficiency, total RNA was isolated after four days of depletion at 25 °C followed by analysis of the target transcript levels. The target analyzed in case of double depletion is indicated at the bottom. (B) The levels of transcripts derived from the endogenous pst gene were analyzed.