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Additional file 20 of Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides

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posted on 2022-08-25, 07:11 authored by Nayeli Escudero Castelán, Dean C. Semmens, Luis Alfonso Yañez Guerra, Meet Zandawala, Mario dos Reis, Susan E. Slade, James H. Scrivens, Cleidiane G. Zampronio, Alexandra M. Jones, Olivier Mirabeau, Maurice R. Elphick
Additional file 20. A. Concentration-response graph comparing the potency/efficacy of S2 (S2.3; EC50 = 2.93 x 10-8 M) and a ‘cocktail’ of neuropeptides derived from the F-type SALMFamide precursor (EC50 = 1.33 × 10-9 M) as ligands for ArKPR6. B. Concentration-response graph comparing the potency and efficacy of S1 (S1.4; EC50 = 4.79 x 10-9 M) and a ‘cocktail’ of neuropeptides derived from the L-type SALMFamide precursor (EC50 = 2.43 × 10-9 M) as ligands for ArKPR7. Each point represents mean values (± S.E.M.) from at least four independent experiments, with each experiment performed in triplicate. Luminescence is expressed as a percentage of the maximal response observed in each experiment. The source data for these experiments are provided in additional file 15.


Leverhulme Trust Biotechnology and Biological Sciences Research Council CONACyT Mexican Council of Science and Technology CONACyT (Mexican Council of Science and Technology)