Additional file 20 of Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides

Additional file 20. A. Concentration-response graph comparing the potency/efficacy of S2 (S2.3; EC50 = 2.93 x 10-8 M) and a ‘cocktail’ of neuropeptides derived from the F-type SALMFamide precursor (EC50 = 1.33 × 10-9 M) as ligands for ArKPR6. B. Concentration-response graph comparing the potency and efficacy of S1 (S1.4; EC50 = 4.79 x 10-9 M) and a ‘cocktail’ of neuropeptides derived from the L-type SALMFamide precursor (EC50 = 2.43 × 10-9 M) as ligands for ArKPR7. Each point represents mean values (± S.E.M.) from at least four independent experiments, with each experiment performed in triplicate. Luminescence is expressed as a percentage of the maximal response observed in each experiment. The source data for these experiments are provided in additional file 15.