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                     Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

Quadros, Rolen; Miura, Hiromi; Harms, Donald; Akatsuka, Hisako; Sato, Takehito; Aida, Tomomi; Redder, Ronald; Richardson, Guy; Inagaki, Yutaka; Sakai, Daisuke; Buckley, Shannon; Seshacharyulu, Parthasarathy; Batra, Surinder; Behlke, Mark; Zeiner, Sarah; Jacobi, Ashley; Izu, Yayoi; Thoreson, Wallace; Urness, Lisa; Mansour, Suzanne; Ohtsuka, Masato; Gurumurthy, Channabasavaiah

doi:10.6084/m9.figshare.c.3781352_D1.v1

13059_2017_1220_MOESM1_ESM.docx (8.91 MB)

Additional file 1: of Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

journal contribution

posted on 2017-05-17, 05:00 authored by Rolen Quadros, Hiromi Miura, Donald Harms, Hisako Akatsuka, Takehito Sato, Tomomi Aida, Ronald Redder, Guy Richardson, Yutaka Inagaki, Daisuke Sakai, Shannon Buckley, Parthasarathy Seshacharyulu, Surinder Batra, Mark Behlke, Sarah Zeiner, Ashley Jacobi, Yayoi Izu, Wallace Thoreson, Lisa Urness, Suzanne Mansour, Masato Ohtsuka, Channabasavaiah Gurumurthy

Twenty-two supplementary figures and one supplementary table. (DOCX 8910 kb)

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National Institute of General Medical Sciences

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Keywords

CRISPR/Cas9 Homology directed repair Easi-CRISPR long ssDNA donors CRISPR ribonucleoproteins Cre-LoxP Conditional knockout Reporter and recombinase knock-in

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CC BY + CC0

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