Additional file 1 of Western diet consumption by host vertebrate promotes altered gene expression on Aedes aegypti reducing its lifespan and increasing fertility following blood feeding

Additional file 1: Table S1. Diet composition. Table S2. Primer list. Figure S1. Mice were fed with either a CHOW or HSHF diet for 20 weeks. Over the course of the experiment, metabolic syndrome was followed by tracking weight gain, glucose, and insulin sensitivity. Then, Aedes mosquitoes were allowed to feed on anesthetized mice, and whole-body relative expression levels of A fatty acid synthase I, B LSD1, C LSD2, D pyruvate kinase, E glycogen synthase, and F PINK1 were measured by qRT-PCR 1 and 4 days pbm. Sugar-fed (SF) mosquitoes were used as a baseline for gene expression. Four biological replicates were prepared using independent mosquito hatches and dietary protocols. A Unpaired t-test and B–F one-way ANOVA followed by Tukey’s multiple comparison tests were performed. ns: non-significant, *P < 0.05. Figure S2. Mice were fed with either a CHOW or HSHF diet for 20 weeks. Over the course of the experiment, metabolic syndrome was followed by tracking weight gain, glucose, and insulin sensitivity. Then, Aedes mosquitoes were allowed to feed on anesthetized mice, and whole-body relative expression levels of A 16S, B catalase, C glutathione peroxidase, and D oxidation resistance 1 (A–D). Sugar-fed (SF) mosquitoes were used as a baseline for gene expression. Four biological replicates were prepared using independent mosquito hatches and dietary protocols. One-way ANOVA followed by Tukey’s multiple comparison tests was performed. ns: non-significant.