13148_2019_617_MOESM1_ESM.pdf (376.94 kB)
Additional file 1: of Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells
journal contribution
posted on 2019-02-04, 05:00 authored by Olivia Cypris, Joana Frobel, Shivam Rai, Julia Franzen, Stephanie Sontag, Roman Goetzke, Marcelo Szymanski de Toledo, Martin Zenke, Wolfgang WagnerFigure S1. Differentiation of iPSCs toward hematopoietic progenitor cells. (a) Phase contrast images in the course of hematopoietic differentiation cultures (on days 0, 6, 10, and 20). Scale bar = 500 μm and scale bar in inlet = 100 μm. (b) Exemplary cytospin analysis on day 22 reveals prevailing monocytic morphology. Scale bar = 10 μm. (c) Flow cytometry analysis of hematopoietic differentiation cultures on days 6 and 20. Blots are representative for three independent experiments. (d) Frequencies of CD31, CD34, CD43, CD45, and cKIT populations on day 6 and 20 of differentiation. Data represent the mean of three independent experiments ± SD. (PDF 376 kb)