Additional file 1 of Taf14 is required for the stabilization of transcription pre-initiation complex in Saccharomyces cerevisiae

Additional file 1: Figure S1. Deletion of TAF14 is lethal in yaf9Δ, rpb4Δ and rpb9Δ cells in W303 background, but functional C-terminal domain of Taf14 rescues rpb4Δtaf14Δ and rpb9Δtaf14Δ lethality. Tetrad analysis following sporulation of AKY1820+AKY1916 (A), AKY1786+AKY1818 (B), AKY718+AKY1818 (C), AKY1850+1938 (D) and AKY719+AKY1850 (E) yeast strains. The tetrads were dissected on YPD medium and plates photographed after 4 days of growth at 30 °C. Figure S2. Expression of C-terminal domain of Taf14 rescues yaf9Δtaf14Δ double-mutant from lethality. Tenfold serial dilutions of cells, where TAF14 in its genomic locus is replaced with gene encoding YEATS-deleted Taf14 protein (taf14ΔYEATS) and combined with YAF9 and SAS5 deletions, were spotted onto SC plates and grown at 30 °C for 2 days. Figure S3. Western blot analysis of Taf14 (A) and Rpb3 (B) in response to Rpb9 depletion. Rpb9 anchor-away strains with wt Taf14 or mutant taf14W81A were incubated with DMSO (+Rpb9) or rapamycin (−Rpb9) for 2 h. Taf14 was detected with anti-Taf14 antibody, RNAPII Rpb3 subunit was C-terminally tagged with E2-tag and detected with 5E11 antibody. A sample from taf14Δ strain expressing Rpb3 without E2-tag was used as a negative control (N). Table S1. Yeast strains.