Additional file 1 of TLR8 escapes X chromosome inactivation in human monocytes and CD4+ T cells

Additional file 1: Table S1. PCR primer pairs used in the preparation of the RNA FISH probes. S1 Data. TLR7 escape from XCI (monocytes, women). S2 Data. RNA FISH analysis of CD14+ monocytes, signals on the Xi. Left, observed frequencies: cell counts cross-classified as 2×2 contingency tables depending on the presence of transcriptional foci for TLR7 or TLR8 on the Xi of monocytes from women and from men with Klinefelter syndrome. Right, the corresponding expected frequencies, computed under the null hypothesis of mutually independent transcription of the two genes in cis on the Xi. Donor IDs in red. S3 Data. RNA FISH analysis of CD14+ monocytes, total cells. Left, observed frequencies: cell counts cross-classified as 2×2 contingency tables depending on the presence of transcriptional foci for TLR7 or TLR8, regardless of Xa markers (Any X plots). Right, the corresponding expected frequencies, computed under the null hypothesis of mutually independent transcription of the two genes. Donor IDs in red. S4 Data. RNA FISH analysis of CD14+ monocytes, Xa-positive cells. Left, observed frequencies: cell counts cross-classified as 2×2 contingency tables depending on the presence of transcriptional foci for TLR7 or TLR8. Only those cells positive for the Xa marker probe (Xa+) were counted. Right, the corresponding expected frequencies, computed under the assumption of mutually independent transcription of the two genes. Donor IDs in red. S5 Data. Xa versus Xi comparison for patterns of TLR7 and TLR8 transcription. The 3 × 2 tables show TLR7+ TLR8+, TLR7− TLR8+, and TLR7+ TLR8− cell counts in the RNA FISH data for the Xa and the Xi of monocytes from female and Klinefelter syndrome male donors; p-values from Monte Carlo χ2 tests with 106 replications. Donor IDs in red. S6 Data. RNA FISH analysis of CD4+ T lymphocytes from women and normal men. Left, observed frequencies: cell counts cross-classified as 2 × 2 contingency tables depending on the presence of transcriptional foci for TLR7 or TLR8. Right, expected frequencies, computed from the observed frequencies under the assumption of mutually independent transcription of the two genes. Donor IDs in red. The any X data (A, C) correspond to cells scored regardless of the hybridization with the Xa marker probe. In the Xa+ data (B, D), only cells positive for the Xa marker probe were scored.