Additional file 1 of Syntrophic acetate oxidation replaces acetoclastic methanogenesis during thermophilic digestion of biowaste

Additional file 1: Figure S1. Relative abundance of hydrogenase classes identified in the metagenome. Figure S2. Relative abundance of metagenomic 16S rRNA gene fragments from PFL9 affiliating with Bacteria and Archaea as shown in Fig. 1a but the highest classification rank of the major groups is depicted. Figure S3. Relative abundance of bacterial and archaeal groups that contributed ≥1% to total 16S rRNA sequences in at least one sample in acetate-fed enrichment cultures. The microbial community was either analyzed by 16S rRNA gene amplicon sequencing (iTags) or metagenome sequencing (Meta). Figure S4. A simplified overview of the metabolic functions reconstructed from Firmicutes MAG-E2. Hyd, [FeFe] hydrogenase group A3; Hdr, heterodisulfide reductase; Rnf, Na+/H+ translocating ferredoxin:NAD+ oxidoreductase; Q, quinone; ETF, electron transfer flavoprotein; ETF-Q OR, electron transfer flavoprotein/quinone oxidoreductase; FDH, formate dehydrogenase; Dsr, dissimilatory sulfite reductase; C, DsrC; Nar, nitrate reductase; Nir, nitrite reductase; Nor, nitric oxide reductase; SDH, succinate dehydrogenase; GH, glycosyl hydrolase; EM, electron mediator; CM, cytoplasmic membrane. Figure S5. Maximum Likelihood (RAxML) tree of dissimilatory sulfite reductase genes (DsrAB) recovered from Firmicutes MAG-E2. Closed circles indicate bootstrap support ≥70%. The reference database used for phylogenetic reconstruction contained 340 sequences of DsrAB proteins (Loy et al., 2008; Environ. Microbiol. 11: 289-299). Figure S6. Microbial community composition in nine full-scale thermophilic biowaste digesters summarized at phylum level. The relative abundance was revealed by 16S rRNA gene amplicon sequencing. Figure S7. Total ammonia nitrogen (g NH4+-N/kg) and pH in PFL1 and PFL8. Table S1. General statistics about MAGs recovered from the reactor PFL9 and from the enrichment culture. Table S2. Overview of the sampled biogas reactors and performed experiments. Table S3. Number of quality trimmed 16S rRNA gene amplicons kept for taxonomic classification. Table S4. Comparison of alpha diversity measures determined for the nine biogas reactors.