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Additional file 1 of Short- and long-term dietary supplementation as well as withdrawal of the enteric methane inhibitor 3-nitrooxypropanol reveal distinct effects on the rumen microbial community

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posted on 2025-12-02, 05:08 authored by Youyoung Choi, Mi Zhou, Atmir Romero-Pérez, Karen A. Beauchemin, Stephane Duval, Maik Kindermann, Le Luo Guan
Additional file 1: Table S1. Ingredient and chemical composition of the basal diet in the short-term study. Table S2. Ingredient and chemical composition of the basal diet in the long-term study. Additional file 2: Table S3. Pairwise comparisons of beta diversity of bacterial, archaeal, and protozoal communities after short-term 3-NOP supplementation in beef cattle. Table S4. Pairwise comparisons of beta diversity of bacterial, archaeal, and protozoal communities after long-term 3-NOP supplementation in beef cattle. Table S5. Effects of short-term 3-NOP supplementation on rumen microbial taxa (bacteria, archaea, and protozoa) based on relative and estimated absolute abundances. Table S6. Effects of long-term 3-NOP supplementation on rumen microbial taxa (bacteria, archaea, and protozoa) based on relative and estimated absolute abundances. Table S7. Effects of long-term 3-NOP supplementation and withdrawal on rumen microbial taxa (bacteria, archaea, and protozoa) based on relative and estimated absolute abundances. Table S8. Effects of short-term 3-NOP supplementation on predicted rumen functions based on CowPI. Table S9. Effects of long-term 3-NOP supplementation on predicted rumen functions based on CowPI. Table S10. Summary of microbial co-occurrence network metrics in the short-term 3-NOP supplementation. Table S11. Summary of microbial co-occurrence network metrics in the long-term 3-NOP supplementation.

Funding

Beef Cattle Research Council Cluster DSM Nutritional Products Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery, and NSERC Alliance program

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    Journal of Animal Science and Biotechnology

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