13068_2021_2079_MOESM1_ESM.pdf (168.78 kB)
Additional file 1 of QTL mapping of a Brazilian bioethanol strain links the cell wall protein-encoding gene GAS1 to low pH tolerance in S. cerevisiae
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posted on 2021-12-17, 04:26 authored by Alessandro L. V. Coradini, Fellipe da Silveira Bezerra de Mello, Monique Furlan, Carla Maneira, Marcelo F. Carazzolle, Gonçalo Amarante Guimaraes Pereira, Gleidson Silva TeixeiraAdditional file 1. Phenotypic distribution of 48 JAY270 segregants growing on solid YPD containing decreasing concentrations of pH to define the Minimum Inhibitory Concentration. A. Boxplot graph containing the colony size values of 48 JAY270 segregants growing on solid YPD at different pH values (4; 3.5; 3; 2.5; 2.1). B. Images of each low pH plate containing the 48 segregants evaluated on this minimum inhibitory concentration assay. The images were captured 96 hours after plating.
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Fundação de Amparo à Pesquisa do Estado de São Paulo
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regarding bioethanol productionmany biotechnological processesdifferent harsh conditionsbulk segregant analysisgas1 sequence alignmentimprove cell viabilitycell wall proteinmajor qtl associatedindustrial strain pedracerevisiae strains pointedlow ph resistancegas1 allele swapresults collectively showoften impact productivityimprove acid resistancecomplex genetic architecturework revealed gas1encoding gene gas1low ph tolerancelow phallele gas1genetic architecturecell exposuresusceptible strainqtl mappingtolerant strainslaboratory strainsgenetic basisyeast cellsusually challengedtype isolatestraditional foodthroughput approachsynonymous mutationrelevant traitsproductivity losspowerful foundationmodern biofuelmainly duelargely appliedlarge numbergeneration ethanolfermentation standfermentation processevaluated phenotypechromosome xcasual variantbiochemicals factoriesbeverage industriesbased highacidic toleranceacidic environments
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