Additional file 1: of Prevalence of porcine circovirus type 3 in pigs in the southeastern Chinese province of Zhejiang

Table S1. Geographical distribution of 283 clinical samples from Zhejiang province of China and PCV3 prevalence as detected by qPCR. Figure S1. Identification of PCV3 capsid protein in E. coli by SDS-PAGE (A) and Western blotting using rabbit anti-PCV3-capsid serum (B) and mouse anti-His monoclonal antibody (C). M: protein marker in KDa. Cap: PCV3-capsid protein purified by Ni2+-NTA affinity column. Table S2. Evaluation of the in-house specificity of the indirect ELISA using PCV3 capsid protein as the coating antigen for differential detection of PCV3 and PCV2 antibodies in 30 PCV2-positive and 30 PCV3 positive serum samples. Table S3. Geographical distribution of pig serum samples from Zhejiang province of China and sero-prevalence of PCV3 infection as determined by indirect ELISA. Table S4. PCV3 strains used for sequence alignment and phylogenetic analysis. (DOCX 2126 kb)