Additional file 1 of MxB impedes the NUP358-mediated HIV-1 pre-integration complex nuclear import and viral replication cooperatively with CPSF6

Additional file 1. MxB inhibits nuclear import and integration of HIV-1 reverse transcripts. (a) Expression of MxB in HeLa cell lines. Location of MxB in HeLa-Ctrl and HeLa-MxB cells was shown in left. HeLa-Ctrl and HeLa- MxB cells were fixed and stained for MxB (red) and DAPI (blue) for cell nuclei. A representative image was depicted. Western blot of the expression of MxB in HeLa-Ctrl and HeLa-MxB cells was shown in right panel. (b) HeLa-Ctrl and HeLa-MxB cells were infected with VSV-G pseudotyped HIV-1 luciferase reporter virus, infectivity was determined 48 h post infection. (c) HeLa-Ctrl and HeLa-MxB cells were incubated with VSV-G pseudotyped HIV-1 luciferase reporter virus, qPCR analysis of HIV-1 Late RT DNA, 2-LTR circles and Integrated DNA was preformed 24 h post infection. Results were a summary of 3 independent experiments,, an unpaired t test was performed (NS, not significant, *p < 0.05, **p < 0.01 and ***p < 0.001). (d) Primary MDMs were isolated from three independent donors and transduced with lentivirus overexpressing MxB. 48 h later, cells were challenged with HIV-1Bal and infectivity was determined 48 h later. (e) Primary CD4+ T cells were isolated from three independent donors and transduced with lentivirus overexpressing MxB. 48 h later, cells were challenged with HIV-1NL4-3 and infectivity was determined 48 h later. The mean ± SD of three technical replicates were shown for each donor.