13024_2021_471_MOESM1_ESM.pdf (3.83 MB)
Additional file 1 of Microglial inclusions and neurofilament light chain release follow neuronal α-synuclein lesions in long-term brain slice cultures
journal contribution
posted on 2021-08-12, 04:00 authored by Melanie Barth, Mehtap Bacioglu, Niklas Schwarz, Renata Novotny, Janine Brandes, Marc Welzer, Sonia Mazzitelli, Lisa M. Häsler, Manuel Schweighauser, Thomas V. Wuttke, Deborah Kronenberg-Versteeg, Karina Fog, Malene Ambjørn, Ania Alik, Ronald Melki, Philipp J. Kahle, Derya R. Shimshek, Henner Koch, Mathias Jucker, Gaye TanriöverAdditional file 1: Supplementary Fig. 1. αS pff characterization. (A, B) Proteolytic profiles of monomeric (A) and fibrillar (B) αS (100 M in PBS) after proteinase K treatment (3.7 μg/μl) at 37 °C. Aliquots were removed from the reaction at the time indicated (in min), immediately denatured with Laemmli buffer at 90 °C for 5 min and analysed on 15% PAGE. The gels were stained with Coomassie blue. The molecular weight markers are shown on the left of each gel. The αS fibrils reveal the typical proteolytic pattern as reported previously [30, 31]. (C, D) Transmission electron micrographs of the αS fibrils before (C) and after fragmentation (D). The scale bar represents 200 nm.
