40168_2021_1193_MOESM1_ESM.pdf (3.95 MB)
Additional file 1 of Metabolite-based dietary supplementation in human type 1 diabetes is associated with microbiota and immune modulation
journal contribution
posted on 2022-01-20, 06:34 authored by Kirstine J. Bell, Sonia Saad, Bree J. Tillett, Helen M. McGuire, Sara Bordbar, Yu Anne Yap, Long T. Nguyen, Marc R. Wilkins, Susan Corley, Shannon Brodie, Sussan Duong, Courtney J. Wright, Stephen Twigg, Barbara Fazekas de St Groth, Leonard C. Harrison, Charles R. Mackay, Esteban N. Gurzov, Emma E. Hamilton-Williams, Eliana MariñoAdditional file 1: Fig. S1. sPLS-DA plot loadings indicating the contribution of each taxa. Fig. S2. Gating Strategy for identification of immune cell phenotypes by CyTOF. Fig. S3. Immune cell phenotype changes following HAMSAB treatment. Fig. S4. Immune cell phenotype changes following HAMSAB treatment. Fig. S5. Circulating pro-inflammatory and anti-inflammatory cytokines measured in subjects at baseline, W6 and W12 following HAMSAB supplementation. Fig. S6. Correlations between concentration of SCFAs, clinical parameters, relative abundance of bacterial communities and significant changes in immune cell subsets. Table S1. Nutritional and gastrointestinal changes across time. Table S2. Functional pathways encoded by the gut microbiota changed after taking dietary supplement. Table S3. Upregulated KEGG pathway gene sets identified using the camera function of limma following 6-weeks of HAMSAB supplementation. Table S4. Results of differential gene expression testing with EdgeR and RUVseq comparing baseline and week 6. Table S5. Mass cytometry panel used for immunophenotyping.
