Additional file 1 of MSMEG_3955 from Mycobacterium smegmatis is a FMN bounded homotrimeric NAD(P)H:Flavin mononucleotide (FMN) oxidoreductase

Additional file 1 : Figure S1. Sequence Alignment of MSMEG_3955 from Mycobacterium smegmatis MC2–155 with Rv3131 from Mycobacterium tuberculosis H37Rv showing Identities: 202/323(63%), Positives: 249/323(77%), Gaps: 0/323(0%). Figure S2. The 1H NMR spectra of FMN oxidised form recorded at 500 MHz, pH -7.6. Figure S3 The 1H NMR spectra of FMN in oxidized (red) and reduced (blue) form recorded at 500 MHz, pH -7.6. Figure S4. Validation of generated monomeric MSMEG_3955. a) PROCHECK gave results as 89.9% in most favoured regions, 8.3% in additional allowed regions, 1.4% in generously allowed regions and 0.4% in disallowed regions. b) Verify 3D gave 96.68% showing the generated trimeric protein model of good quality. c) ERRAT gave overall quality factor 90.7 expressed as the percentage of the protein for which the calculated error value falls below 95% rejection limit. Figure S5. Absorbance-time curve for the reaction of Protein + NADPH + FMN.