Additional file 1 of Lipid A-Ara4N as an alternate pathway for (colistin) resistance in Klebsiella pneumonia isolates in Pakistan

Additional file 1: Table S1. Primers and probe sequences to target the plasmid-mediated colistin resistance (mcr-1) and housekeeping gene rho. Figure S1. Amplification of mcr-1 gene in a positive control NCTC 13846 E. coli strain. DNA was extracted from the pure culture of NCTC 13846 E. coli strain using DNA extraction kit (Qiagen) as per manufacturers instruction. Extracted DNA was subjected to PCR using sequence specific primers of mcr-1 gene. The figure shows the amplification of mcr-1 gene in the positive control used in the experiment. PC denotes positive control and NTC denotes non-template control.