Additional file 1 of Large-scale bioreactor production of extracellular vesicles from mesenchymal stromal cells for treatment of acute radiation syndrome

Additional file 1. Fig. S1: EV and LPS-EV particle yields per mL of conditioned media from flasks versus bioreactor. The mean particle yield per mL (± SEM) were generated from multiple flask production runs isolated from flasks (24H Flask) and LPS EVs (24H+LPS flask) (N=10 biological replicates) from three (F1_F3) MSC isolates. The bioreactor EVs (24H-96H Bioreactor) were generated from multiple production runs (N=4 biological replicates) or the bioreactor LPS-EVs (24H+LPS Bioreactor) after one 24-hours of LPS stimulation run from one B MSC isolate. There was a significant (t-test) increase (p ≤ 0.05) in yield per mL produced in the bioreactor runs for EVs (24H-96H Bioreactor) compared to the respective flask runs. Table S1. Effect of EV or LPS-EV Treatment on Complete Blood Counts after in Mice after Lethal Irradiation. Key: n/a = not applicable. * = p < 0.05, ** = p < 0.01, *** = p < 0.001 as compared to pre-radiation (pre-rad control). Mean CBCs ( +/- SEM) after single i.v. treatment of vehicle (PBS), of EVs made in flasks (Flask-EVs) or bioreactor (Bioreactor-EVs) and EVs from LPS-primed MSCs made in flasks (Flask LPS-EVs) or bioreactor (Bioreactor LPS-EVs).