13287_2023_3549_MOESM1_ESM.pdf (224.67 kB)
Additional file 1 of Lactate: an alternative pathway for the immunosuppressive properties of mesenchymal stem/stromal cells
journal contributionposted on 2023-11-20, 05:40 authored by Carolina Pradenas, Noymar Luque-Campos, Karina Oyarce, Rafael Contreras-Lopez, Felipe A. Bustamante-Barrientos, Andrés Bustos, Felipe Galvez-Jiron, María Jesús Araya, Catalina Asencio, Raúl Lagos, Yeimi Herrera-Luna, Daouda Abba Moussa, Charlotte Nicole Hill, Eliana Lara-Barba, Claudia Altamirano, Alexander Ortloff, Yessia Hidalgo-Fadic, Ana María Vega-Letter, María de los Ángeles García-Robles, Farida Djouad, Patricia Luz-Crawford, Roberto Elizondo-Vega
Additional file 1: Fig. S1. L-Lactate did not induce apoptosis of CD4+ T cells. Representative flow cytometry plots using Annexin V/PI staining for apoptosis (A). CD4+ T cells were treated for 3 days with different L-Lactate concentrations (10 mM, 20 mM and 50 mM) and then stained with Annexin V/PI for flow cytometric analysis. Percentage of live CD4+ T cells after L-lactate incubation (B). Percentage of apoptotic cells after treatment with different L-Lactate concentrations (C). After 3 days of culture with L-Lactate, Treg induction from Th1 (D) and Th17 (E) cells was analyzed by FACS. Results represent the mean ± SD of two independent experiments and four biological samples for CD4+ T cells; *p < 0.05 (unpaired Kruskal–Wallis test). Unless otherwise indicated, comparisons were with control conditions, or with Th1 or Th17 cells.