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Additional file 1 of Investigating the potential effects of selective histone deacetylase 6 inhibitor ACY1215 on infarct size in rats with cardiac ischemia-reperfusion injury

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posted on 17.03.2020, 04:40 by Chao-Feng Lin, Kai-Cheng Hsu, Wei-Chun HuangFu, Tony Eight Lin, Han-Li Huang, Shiow-Lin Pan
Additional file 1: Supplementary Figure 1. Protocol of MPT0E028 administration in ISO-treated rats. BID = twice per day; CTL = control; DMSO = dimethyl sulfoxide; IP = intraperitoneal; ISO = isoproterenol; PO = per os (by mouth); QD = once per day. Supplementary Figure 2. The serum levels of NT-proBNP and collagen area in myocardium of ISO-treated rats. MPT0E028 administration significantly reduced the serum NT-proBNP levels and collagen area in myocardium in ISO-treated rats. BID = twice per day; CTL = control; ISO = isoproterenol; NT-proBNP=N-terminal prohormone of brain natriuretic peptide; QD = once per day. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Supplementary Figure 3. HDAC6 activities in hypoxia-incubated H9c2 cell. Hypoxia-incubated H9c2 cells showed a decreased expression of acetyl-α-tubulin (AC-tubulin) compared to normoxia-incubated H9c2 cells, indicating the increase of HDAC6 activities after hypoxic stress. AC-tubulin = acetyl-α-tubulin; H = hour; H = hypoxia; HDAC = histone deacetylase; min = minutes; N = normoxia.


Ministry of Science and Technology, Taiwan