12864_2021_8206_MOESM1_ESM.pdf (159.97 kB)
Additional file 1 of InMut-finder: a software tool for insertion identification in mutagenesis using Nanopore long reads
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posted on 2021-12-20, 04:11 authored by Rui Song, Ziyao Wang, Hui Wang, Han Zhang, Xuemeng Wang, Hanh Nguyen, David Holding, Bin Yu, Tom Clemente, Shangang Jia, Chi ZhangAdditional file 1: Supplemental Figure S1. Study design for multiple barcoding in one single Nanopore flowcell. BC01 ~ BC13 indicates the barcodes in Nanopore sequencing, and a total of 56 samples, a1 ~ a7, b1 ~ b7, c1 ~ c7, d1 ~ d7, e1 ~ e7, f1 ~ f7, g1 ~ g7, and h1 ~ h7, are pooled in 13 barcodes. Each sample presents twice in one flowcell.
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soybean mutants basedlink observed phenotypehttps :// githubinsertion sites discoveredautomated insertion detection100 insertion sitessite dna fragmentsreverse genetic studiesoxford nanopore sequencingefficient software toolsfinder ”), basednanopore long readssequencing readslong readsinsertion identificationenriched insertionsoftware toolreverse geneticsgenome dnawide insertionsvaluable tooltarget sequencessource codeshell scriptssequences insertedpowerful toolpcr experimentsmedicago truncatulafunctional genomicsflanking sequencesfinder succeededfinder makecrucial rolebioinformatics tool
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