Additional file 1 of Horizontally acquired antibacterial genes associated with adaptive radiation of ladybird beetles

Additional file 1: Figure S1-S9, Tables S1-S7. Figure S1. Flanking genes of the eukaryotic cwh genes predicted from five high-quality genomes. Figure S2. Guanine-Cytosine contents of cwh coding sequences of the ladybird Cryptolaemus montrouzieri. Figure S3. Spatial expression of ladybird cwh genes. Figure S4. Temporal expression of ladybird cwh genes. Figure S5. Expression of ladybird cwh genes in response to bacterial infection. Figure S6. Phylogenetic tree of eukaryotic cwh genes identified from NCBI whole genome shotgun assemblies. Figure S7. Phylogenetic tree of Coccinellidae and their Coleoptera outgroups. Figure S8. Divergence time of Coccinellidae and their Coleoptera outgroups. Figure S9. Number of immunity-related genes in the published Coleoptera genomes. Table S1. Experimental design of cwh genes from ladybird genomes or transcriptomes. Table S2. Primers for ladybird cwh genes used for quantitative PCR. Table S3. Transcriptome data used in this study. Table S4. Information of the putative eukaryotic cwh genes detected from NCBI whole genome shotgun assemblies. Table S5. Estimation of selection pressures of cwh1 and cwh2 across Coccinellinae. Table S6. Primers for ladybird cwh used for cloning. Table S7. Primers for constructing cwh-RNAi strains of the ladybird Cryptolaemus montrouzieri.