Additional file 1 of Hepatitis E virus seroprevalence in pets in the Netherlands and the permissiveness of canine liver cells to the infection

Additional file 1: Table S1. qRT-PCR primer sequences. Figure S1. qRT-PCR determined standard curve. HEV plasmid based standard curve is generated by plotting the log copy number versus the cycle threshold (CT) value. Figure S2. Potent anti-HEV activity of ribavirin and interferon-α (IFN-α) in Huh7.5 cell model. Treatment of ribavirin or IFN-α for 24, 48, 72 or 96 h in the subgenomic model determined by luciferase activity (mean ± SEM, n = 5. CTR, non-treatment control.), and in the infectious model determined by viral RNA (mean ± SEM, n = 4. CTR, non-treatment control). *P < .05; **P < .001; ***P < .0001. Figure S3. Immunofluorescence staining of viral protein ORF2 (red) in BDE cells, upon infection of 24 h, 48 h, 72 h and 96 h. BDE-HEV cells incubated with the matched IgG control antibody serves as negative control, and HEV infected Huh7.5 cells serves as positive control. DAPI (blue) was applied to visualize nuclei. (40× oil immersion objective; Scale bar, 200 μm).