Additional file 1 of Gut microbiota from green tea polyphenol-dosed mice improves intestinal epithelial homeostasis and ameliorates experimental colitis

Additional file 1: Figure S1. Rectal EGCG had a minimum effect on alleviating DSS-induced colitis. (a) Diagram illustrating the mouse model of colitis employed in this study. Rectal PBS and EGCG treatments were indicated. (b) Kinetics of DAI scores throughout the entire duration of the study. (c) Daily body weight changes throughout the entire duration of the study. Data were presented as Means ± SEM (n = 7 per group). Statistical significance was determined using one-way ANOVA, followed by Turkey test. ** P ≤ 0.01, *** P ≤ 0.001 relative to Rectal-CON group; ## P ≤ 0.01, ### P ≤ 0.001 relative to DSS + Rectal-PBS group. (d) Macroscopic pictures of colons and (e) the lengths of colon from each group (n = 7 per group). (f) Histological scores of colons (n = 6 per group) and (g) H&E stained colon sections. Concentrations of four representative pro-inflammatory cytokines, IL-1β (h), IL-6 (i), IL-8 (j), and TNF-α (k) in the plasma. Concentrations of MPO (l), IL-6 (m), and TNF-α (n) in the colon. Data were presented as Means ± SEM (n = 7 per group). Statistical significance was determined using one-way ANOVA, followed by Turkey test. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.