Additional file 1 of Gut microbiota, blood metabolites, and left ventricular diastolic dysfunction in US Hispanics/Latinos

Additional file 1: Supplementary methods: Supplementary Method 1. Study design and population. Supplementary Method 2. Assessment of LVDD. Supplementary Method 3. Gut microbiome profiling. Supplementary Method 4. Metabolome profiling. Supplementary Method 5. Statistical analysis. Supplementary figures: Fig. S1. Overview of the study design and main analyses. Fig. S2. Algorithm for assessing left ventricular diastolic dysfunction (LVDD). Fig. S3. Pooled results of associations between 9 species selected by ANCOM-II and prevalent LVDD in both discovery (ECHORC-SOL) and validation (ECHO-SOL) sets. Fig. S4. Abundances of the identified LVDD-associated gut bacteria species across LVDD grades. Fig. S5. Comparisons of associations between metabolites and prevalent (A) and incident (B) LVDD across models. Fig. S6. A summary of KOs associated with species. Fig. S7. Partial correlation among highlighted LVDD associated species, species associated KOs, and incident LVDD associated metabolites.