Additional file 1 of Functional correlation of ATP1A2 mutations with phenotypic spectrum: from pure hemiplegic migraine to its variant forms

Additional file 1: Supplementary Figure 1. Representative images of transfection efficiency of wild-type and mutant constructs with 100μm scales. Supplementary Figure 2. Survival assays in HeLa cells. The survival rate of each mutant was significantly reduced compared to the wild-type (R65W: P = 0.032, other mutants: P < 0.0001, two-way ANOVA). Supplementary Figure 3. Survival assays in HEK293T cells. The survival rate of each mutant was significantly reduced compared to the wild-type (R65W: P = 0.016, other mutants: P < 0.0001, two-way ANOVA). Supplementary Figure 4. Survival assays in HeLa cells in the absence of ouabain. The survival rates of cells in each mutant group were similar to those in the WT group (P > 0.05, two-way ANOVA). Supplementary Table 1. Clinical characteristics of previously published patients with studied ATP1A2 mutations.