12964_2016_128_MOESM1_ESM.pdf (598.55 kB)
Additional file 1: of Feedback activation of neurofibromin terminates growth factor-induced Ras activation
journal contribution
posted on 2016-02-09, 05:00 authored by Anne Hennig, Robby Markwart, Katharina Wolff, Katja Schubert, Yan Cui, Ian Prior, Manuel Esparza-Franco, Graham Ladds, Ignacio RubioEGF-induced electrophoretic mobility shift of Sos is sensitive to MEK (10 μM UO126) and Erk (50 μM FR180204) but not Rsk (10 μM BI-D1870) or PI3K (100nM Wortmannin) inhibition. HeLa cells pre-treated with the indicated inhibitors or subjected to siRNA-mediated knockdown of neurofibromin (siNF1) were deprived of serum overnight and challenged 30 min with EGF. Lysates were processed for immunodetection of Sos, Rsk1/2/3 and Erk. (PDF 598 kb)
