40168_2019_624_MOESM1_ESM.docx (2.8 MB)
Additional file 1: of Enterobacteriaceae dominate the core microbiome and contribute to the resistome of arugula (Eruca sativa Mill.)
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posted on 2019-01-29, 05:00 authored by Tomislav Cernava, Armin Erlacher, Jung Soh, Christoph Sensen, Martin Grube, Gabriele BergTable S1. Sample and processing details. Composite samples were obtained from multiple plants that were homogenized prior to DNA extractions and isolation of bacteria. Table S2. Alignment of quality-filtered reads to genomes of the Brassicacea plant family. Table S3. Assembly statistics for the Velvet-based de novo assembly. For the median coverage, N50, and Maximum contig length columns two values are shown. The first value for median coverage represents the number of k-mers, while the second value represents the number of utilized bases. Table S4. Multi-resistant isolates identified in antibiotic susceptibility experiments. Colors indicate the isolation source of the isolates. Soil (gray); Rhizosphere (blue); Phyllosphere (green). Figure S1. Metadata profile starting 14âdays before sampling of plants for the metagenome sequencing. Figure S2. Core OTUs assigned to enterobacteria across 50% - 100% of the samples. Figure S3. Neighbor joining tree with max. Sequence identity 0.1 (90% similarity) and RefSeq BLAST assignments. OTU 52 is highlighted in yellow. Figure S4. Neighbor joining tree with max. Sequence identity 0.1 (90% similarity) and RefSeq BLAST assignments. OTU 58 is highlighted in yellow. Figure S5. Neighbor joining tree with max. Sequence identity 0.1 (90% similarity) and RefSeq BLAST assignments. OTU27 is highlighted in yellow. (DOCX 2866 kb)
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Figure S 4. NeighborsimilarityFigure S 2. Core OTUsMaximum contig length columnscore microbiomeDOCX 2866 kbDNA extractionsFigure S 1. Metadata profileFigure S 5. Neighborprocessing detailsTable S 3. Assembly statisticsComposite samplesisolation sourceOTU 52Brassicacea plant familyOTU 58RefSeq BLAST assignmentsantibiotic susceptibility experimentsOTU 27metagenome sequencingFigure S 3. Neighbor0.1Table S 2. AlignmentTable S 4. Multi-resistantmaxcoverageidentitySequence
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