Additional file 1 of Enhanced enzymatic production of cholesteryl 6ʹ-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori
posted on 2021-10-28, 03:28authored bySasikala Muthusamy, Hau-Ming Jan, Ming-Yen Hsieh, Soumik Mondal, Wen-Chun Liu, Yi-An Ko, Wei-Yuan Yang, Kwok-Kong Tony Mong, Guang-Chao Chen, Chun-Hung Lin
Additional file 1. Fig. S1. Chemical structures of CG-MAN, CAG-MAN, and CPG-MAN. Fig. S2. Inhibition of cholesterol biosynthesis abolishes the internalization of CAG-MAN and CPG-MAN by AGS cells. Fig. S3. Co-localization of puncta of CAG-MAN and CPG-MAN with intracellular H. pylori 26695. Fig. S4. The autophagy response induced by ΔCGT in the presence of CG, CAG, or CPG. Fig. S5. Lysosomal pH and calcium levels remained unaffected by the infection with H. pylori 26695 or ΔCGT and the treatment with bafilomycin A1 increased the number of intracellular H. pylori.
Funding
Academia Sinica Ministry of Science and Technology, Taiwan