Additional file 1 of Engineering marine fungi for conversion of d-galacturonic acid to mucic acid

Additional file 1: Table S1. List of primers used. Figure S1. The fungal pathway for d-galacturonic acid metabolism (reactions 1–4) and the reactions (5–6) necessary to produce mucic acid from d-galacturonic acid. The enzyme are: (1) d-galacturonate reductase EC 1.1.1.365, (2) l-galactonate dehydratase EC 4.2.1.146, (3) 2-keto-3-deoxy-galactonate aldolase EC 4.1.2.54 and (4) l-glyceraldehyde reductase EC 1.1.1.372, (5) d-galacturonate (uronate) dehydrogenase EC 1.1.1.203, (6) lactonase or spontaneous opening. (See Richard P, Hilditch S. d-Galacturonic acid catabolism in microorganisms and its biotechnological relevance. Appl Microbiol Biotechnol. 2009; 82:597–604 and Kuivanen J, Biz A, Richard P. Microbial hexuronate catabolism in biotechnology. AMB Expr 201; 9:16 for references and review of other pathways of d-galacturonic acid metabolism, including details on the enzymatic opening of the galactarolactone to the linear mucic acid, reaction 6.) Figure S2. The gar2 sequences obtained for Trichoderma sp. LF328 and Coniochaeta sp. MF729 gar2 gene.