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Additional file 1: of Dynamic silencing of somatic L1 retrotransposon insertions reflects the developmental and cellular contexts of their genomic integration
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posted on 2017-05-10, 05:00 authored by Manoj Kannan, Jingfeng Li, Sarah Fritz, Kathryn Husarek, Jonathan Sanford, Teresa Sullivan, Pawan Tiwary, Wenfeng An, Jef Boeke, David SymerFigure S1. Schematic of beta-lactamase reporter assay. Figure S2. Schematics of newly integrated sequences retrotransposed by L1. Figure S3. Standardized blue and green cell populations as controls for flow cytometry analysis. Figure S4. Dense maintenance methylation of pre-existing L1 retrotransposons in cultured human colorectal cancer (HCT116) cells is reduced dramatically in DNMT1 and DNMT3b methyltransferase double knockout cells. Figure S5. Lack of cytosine methylation at silenced, de novo L1 reporter insertions in cultured HeLa cells. Figure S6. Variable L1 reporter expression in cultured cancer cells is associated with changes in histone acetylation. Table T1. Oligonucleotides used in this study. Table T2. De novo L1 integrant features. Table T3. Expression status of predicted SAGE tags from consensus human L1 template sequence in sense and antisense orientation. (PDF 18544 kb)
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knockout cellsTable T 2. Desequences retrotransposedSAGE tagsL 1 retrotransposonsDNMT 3b methyltransferaseDNMT 1histone acetylationbeta-lactamase reporter assaycell populationsPDF 18544 kbFigure S 5. Lackcancer cellsFigure S 2. SchematicsL 1 template sequenceFigure S 6. Variable L 1 reporter expressionantisense orientationcolorectal cancerHCTHeLa cellsTable T 1. Oligonucleotidesgenomic integration Figure S 1. SchematicL 1 integrant featuresflow cytometry analysiscytosine methylationL 1 retrotransposon insertionsTable T 3. Expression statusL 1. Figure S 3.L 1 reporter insertionsFigure S 4. Dense maintenance methylation
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