13064_2020_141_MOESM1_ESM.pdf (134.36 kB)
Additional file 1 of Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function
journal contribution
posted on 2020-03-18, 04:46 authored by Elizabeth M. McNeill, Cheryl Thompson, Brett Berke, Vivian T. Chou, Jannette Rusch, April Duckworth, Jamin DeProto, Alicia Taylor, Julie Gates, Frank Gertler, Haig Keshishian, David Van VactorAdditional file 1 Supplemental Figure 1. Fluorescence images (A-F) and quantification (G-H) of NMJs from muscle 6/7 in segment A2 of third-instar wandering larvae. Wild-type flies (control; A-A") and Abl mutants (Abl2/Df Ste34; D-D") are shown stained with horseradish peroxidase (HRP; green, left panels), Futsch (red, middle panels), and with the HRP/Futsch channels merged (yellow, right panels). Staining with active zone markers Brp and endophilin (Endo) as well as the postsynaptic marker glutamate receptor subunit III (GluRIII) was qualitatively normal (B,C,E,F). G, Quantification of synaptic 1b and 1 s bouton number at muscle 4 (orange bars) and muscle 6/7 (blue bars). Abl mutant lines demonstrate an increase in bouton number relative to wild-type control (G). H, Branch number is also increased in abl mutants (E). Error bars indicate ± s.e.m. of genotype; orange and blue shading indicate ± s.e.m. of muscle 4 and 6/7 controls, respectively; n ≥ 20 NMJs for all genotypes, scale = 200 μm.