Springer Nature
Browse
13064_2020_141_MOESM1_ESM.pdf (134.36 kB)

Additional file 1 of Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function

Download (134.36 kB)
journal contribution
posted on 2020-03-18, 04:46 authored by Elizabeth M. McNeill, Cheryl Thompson, Brett Berke, Vivian T. Chou, Jannette Rusch, April Duckworth, Jamin DeProto, Alicia Taylor, Julie Gates, Frank Gertler, Haig Keshishian, David Van Vactor
Additional file 1 Supplemental Figure 1. Fluorescence images (A-F) and quantification (G-H) of NMJs from muscle 6/7 in segment A2 of third-instar wandering larvae. Wild-type flies (control; A-A") and Abl mutants (Abl2/Df Ste34; D-D") are shown stained with horseradish peroxidase (HRP; green, left panels), Futsch (red, middle panels), and with the HRP/Futsch channels merged (yellow, right panels). Staining with active zone markers Brp and endophilin (Endo) as well as the postsynaptic marker glutamate receptor subunit III (GluRIII) was qualitatively normal (B,C,E,F). G, Quantification of synaptic 1b and 1 s bouton number at muscle 4 (orange bars) and muscle 6/7 (blue bars). Abl mutant lines demonstrate an increase in bouton number relative to wild-type control (G). H, Branch number is also increased in abl mutants (E). Error bars indicate ± s.e.m. of genotype; orange and blue shading indicate ± s.e.m. of muscle 4 and 6/7 controls, respectively; n ≥ 20 NMJs for all genotypes, scale = 200 μm.

Funding

National Institute of Neurological Disorders and Stroke National Institutes of Health

History

Usage metrics

    Neural Development

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC