Additional file 1 of Development of a genetic engineering toolbox for syngas-utilizing acetogen Clostridium sp. AWRP

Additional file 1: Supplementary Methods; Fig. S1. Transformation of unmethylated pKLJM082 and pKLJM083; Fig. S2. Schematic diagram of pyrE deletion in AWRP and confirmation of the genotype and phenotype; Table S1. Strains and plasmids used in this study; Table S2. Primers used in this study; Table S3. Guide RNA sequences for genome editing; Sequence of the synthesized guide RNA cassette for AsCas12a (cloned in pUC57)