Additional file 1: of Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus
journal contributionposted on 02.12.2015 by Yang Yang, Xiaodong Qin, Guangxiang Wang, Yuen Zhang, Youjun Shang, Zhidong Zhang
Any type of content formally published in an academic journal, usually following a peer-review process.
Figure S1. Three forward primes (F1-F3), one probe and three reverse primers (R1-R3) were tested to select combinations yielding the highest analytical RPA sensitivity. The amplification results of nine different combination of primers with the exo probe ORFV RPA P (i.e. 1: F1/R1, 2: F2/R2, 3: F1/R2, 4: F2/R1, 5: F1/R3, 6: F2/R3, 7: F3/R1,8: F3/R2,9: F3/R3, 10: NC) were shown in the figure. Standard DNA of 104 genome copies were used as template. (DOCX 24 kb)