40659_2024_501_MOESM1_ESM.pdf (535.56 kB)
Additional file 1 of Connexin channels and hemichannels are modulated differently by charge reversal at residues forming the intracellular pocket
journal contribution
posted on 2024-05-24, 03:40 authored by Felipe Villanelo, Peter J. Minogue, Jaime Maripillán, Mauricio Reyna-Jeldes, Joaquin Jensen-Flores, Isaac E. García, Eric C. Beyer, Tomás Pérez-Acle, Viviana M. Berthoud, Agustín D. MartínezAdditional file 1: Figure S1. Temporal course of fluctuations during the simulations. Left panel, Graph shows the time-course of root mean square deviation (RMSD) for wild-type and mutant Cx50 hemichannels in a 100-ns simulation. Right panel, Graph shows the root mean square fluctuation (RMSF) around each amino acid residue (aa) for wild-type and mutant Cx50 hemichannels in a 100-ns simulation. The vertical shades mark the position of the amino acid residues belonging to the N-terminal helix (mauve) and the transmembrane domains (light mint green). Data are presented as mean (central line) ± standard deviation (shade) from three independent 100-ns simulations ran for wild-type Cx50 (green), Cx50R33E (light blue), Cx50E162R (orange), Cx50E162Q (yellow) and Cx50R33E,E162R (purple) hemichannels.
