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Additional file 1 of Cis-regulatory analysis of Onecut1 expression in fate-restricted retinal progenitor cells

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posted on 20.03.2020 by Sruti Patoori, Nathalie Jean-Charles, Ariana Gopal, Sacha Sulaiman, Sneha Gopal, Brian Wang, Benjamin Souferi, Mark M. Emerson
Additional File 1. Genomic map of potential regulatory elements identified near Onecut1 gene locus. Coding regions of Onecut1 and WDR72 from multiple species are marked by blue bars. The 5′ most transcriptional start site for Fam214a begins in the ACR10 region, as determined by examination of previous retinal transcriptome datasets [6], and Fam214a transcripts extend to the right. Yellow bars and lines at the bottom indicate sequence conservation between chick and mouse (mm10 assembly) genomes. Chromatin accessibility reads from the ThrbCRM1-positive population are shown in teal, reads from the ThrbCRM1-negative cell population are shown in magenta. Peaks in magenta may be cut off as the data was scaled to optimally visualize the ThrbCRM1 chromatin accessibility. Black bars at the top indicate potential regulatory elements, labelled as Evolutionary Conserved Region (ECR) if originally identified through sequence conservation and Accessible Chromatin Region (ACR) if identified through ATAC-seq data. Elements which could not be cloned or assayed for activity are marked with an asterisk.

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National Science Foundation Alfred P. Sloan Foundation National Institutes of Health

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