Additional file 1 of Cholangiocyte organoids to study drug-induced injury

Additional file 1: Table S1. List of primers for gene expression analyses. Table S2. List of antibodies for immunofluorescence. Figure S1. Cytotoxicity of BA cocktail in cholangiocyte-like cell organoids (CLCOs). LDH release as cytotoxicity read-out after exposure of CLCOs to various concentrations of the BA cocktail (for composition see Table 1 of the main manuscript) for 24 and 72 h. Data are presented as mean ± SD of five independent donors. Statistical differences between groups were determined using one-way ANOVA followed by Tukey’s test for multiple comparisons; *P < 0.05. Figure S2. Effects of CPZ and BA cocktail on the proinflammatory gene expression. A, B CLCOs were pretreated with solvent (control), 30 μM CPZ with or without BA cocktail (BA 40× or 80×) for 24 (A) and 72 h (B). Gene expressions were estimated by qPCR and normalized to the housekeeping genes. Data are presented as mean ± SD of five independent donors. Statistical differences between groups were using one-way ANOVA followed by Tukey’s test for multiple comparisons; *P < 0.05. Figure S3. Effects of CPZ and BA cocktail on the proinflammatory release. A, B CLCOs were incubated with 30 μM CPZ with or without BA cocktail (BA 40× or 80×) and medium were estimated by ELISA, IL6 and IL8 were tested by 24 (A) and 72 h (B). Data are presented as mean ± SD of five independent donors. Statistical differences between groups were using one-way ANOVA followed by Tukey’s test for multiple comparisons.