Additional file 1 of Biocatalytic cascade to polysaccharide amination

Additional file 1: Figure S1. Acetophenone evaporation at 37 °C. Figure S2. The initial activity of CvATA and SpATA. Figure S3. Absorption spectra of red precipitates formed in the Q-NPEA assay. Figure S4. HPLC results confirming pyruvate depletion. Figure S5. Gel formation by acetyl bond formation in oxidized intermediates. Figure S6. Docking of galactomannan oligosaccharide on SpATA surface before and after alanine substitutions. Figure S7. PGBC in SpATA hosting the PLP cofactor. Figure S8. Discoloration of PLP catalyzed by CvATA. Figure S9. Formation of PMP-derived compounds in the discolored PLP solution. Figure S10. Initial rates of the wild-type SpATA and SpATA E407A measured on pyruvate with varying concentrations. Figure S11. The location of E407A mutation near the interfacial loops. Figure S12. Galactose oxidase inhibition by the aminated product measured using the ABTS assay. Table S1. A list of primers used in the site-directed mutagenesis of SpATA.