Additional file 1 of Automation assisted anaerobic phenotyping for metabolic engineering

Additional file 1: Table S1. Names and formulae of reaction abbreviations mentioned in Fig. 7 (main text). Metabolites in the reaction formulae are represented by their BiGG ID. Figure S1. Change in sterility with air-gap (data consolidated from main text Fig. 2d). Figure S2. Increase in pipetting error upon increasing pipetting speed by 300% for different volume ranges. Figure S3. Distribution of biomass yields (ratio of final to initial biomass) of wild type E. coli MG1655 grown in Rich Defined Media with different seals. Figure S4. Time-course showing cell density and instantaneous growth rate of different E. coli strains (described in Materials & methods) in RDM with and without a layer of oil in the presence of oxygen and with a layer of mineral oil inside an anaerobic chamber. Figure S5. Time-course showing cell density and instantaneous growth rate of E. coli strains (described in Materials & methods) with different pre-culturing strategies. Figure S6. Variance explained by each principal component for principal component analysis performed on metabolite yields and growth rates of E. coli strains (described in Materials & methods) grown in rich defined media in a bioreactor and microplates supplemented with reducing agents. Figure S7. Principal component analysis performed on the metabolite yields and growth rates of E. coli strains (described in Materials & methods) grown in rich defined media in a bioreactor and microplates supplemented with reducing agents. Figure S8. A comparison E. coli's metabolite yields and growth rates obtained from a bench-top 0.5 L bioreactor and 96-well microplates with different reducing agents for the strains: a. Wild Type MG1655, b. MG1655 Δ(adhE; pta)-D1, c. MG1655 Δ(adhE; pta)-D28, and d. MG1655 Δ(adhE; pta)-D59. Figure S9. Principal component analysis performed on the metabolite yields and growth rates of E. coli strains (described in Materials & methods) grown in rich defined media in a bioreactor and microplates supplemented with different substrate concentrations. Figure S10. A comparison E. coli's metabolite yields and growth rates obtained from a bench-top 0.5 L bioreactor and 96-well microplates with different initial glucose concentrations for the strains: a. Wild Type MG1655, b. MG1655 Δ(adhE; pta)-D1, c. MG1655 Δ(adhE; pta)-D28, and d. MG1655 Δ(adhE; pta)-D59.