12934_2020_1418_MOESM1_ESM.pdf (829.99 kB)
Additional file 1 of Activation and enhancement of caerulomycin A biosynthesis in marine-derived Actinoalloteichus sp. AHMU CJ021 by combinatorial genome mining strategies
journal contribution
posted on 2020-08-07, 03:51 authored by Yunchang Xie, Jiawen Chen, Bo Wang, Tai Chen, Junyu Chen, Yuan Zhang, Xiaoying Liu, Qi ChenAdditional file 1: Additional file 1: Table S1. Genome features of Actinoalloteichus sp. AHMU CJ021; Table S2. Number of genes associated with the general COG functional categories; Table S3. The biosynthetic gene clusters of secondary metabolites in Actinoalloteichus sp. AHMU CJ021 analyzed by antiSMASH 5.0; Table S4. Deduced functions of the open reading frames (ORFs) indicated in Fig. 2; Table S5. Fermentation media used for caerulomycin A (CRM A) production; Table S6. The mutants obtained from ribosome engineering experiments; Table S7. The CRM A production comparison of three camE-expressing mutants; Table S8.1H NMR and 13C NMR spectral data of CRM A in DMSO-d6; Table S9. The comparison of selected mutants generated from UV mutagenesis; Table S10. CRM A production titer of optimal mutant XC-11GUR; Table S11. The dose of all factors in medium N2 by using Plackett-Burman Design; Table S12. Screening of significant variables for CRM A production in Medium N2 by using Plackett-Burman Design; Table S13. The effects of all factors of Medium N2 for CRM A production by using Plackett-Burman Design; Table S14. The dose of important factors in response surface analysis; Table S15. The design of experiments and response of CRM A production; Table S16. The primers used in identification of gentamycin-resistant mutant; Table S17. The primers used in gene expression analysis; Fig. S1. Phylogenetic tree of Actinoalloteichus sp. AHMU CJ021; Fig. S2. HR-ESI-MS spectrum of CRM A; Fig. S3.1H NMR spectrum of CRM A in DMSO-d6; Fig. S4.13C NMR spectrum of CRM A in DMSO-d6; Fig. S5. CRM A production comparison of different generations of mutants; Fig. S6. The quantitative HPLC standard curves.