Additional file 1 of A tobamovirus RNA motif and TMV-Hel cooperatively suppress the host defense factor TRX

Additional file 1: Figure S1. Mining of active EL1 elements from tobamoviruses. Figure S2. In vivo verify the BiFCrp method by testing the interaction of TMV UPD with EF1A. Figure S3. Transcriptional- and protein-level analyses of the constructs in BiFC and BiFCrp related experiments. Figure S4. In vivo detecting the interaction of BPMV-EL1 with NbTRXh1 by BiFCrp. Figure S5. Effect of NbTRXh1-OE or -RNAi prior to pAT-BPMV-EL1-CP -inoculation on virus symptom in N. benthamiana. Figure S6. TMV-induced expression of NbTRXh1 and its subcellular localization. Figure S7. BPMV-EL1b and the redox site of NbTRXh1 are crucial for their interaction. Figure S8. The interaction of TMV-Hel with both BPMV-EL1 motif and NbTRXh1. Figure S9. The influence of TMV-Hel on GFP-expression. Figure S10. No effect of NbTRXh1 was detected on the accumulation of TMV-Hel. Figure S11. RNA-EMSA assay of the binding activity of NbTRXh1 to different tobamovirus-EL1 motifs. Figure S12. Comparative analysis of BPMV-EL1 and TMV-EL1 motifs on NbTRXh1 activity. Figure S13. Comparative analysis of the activities of BPMV-EL1 and its mutants BPMV-EL1a-c in viral GFP-expression.