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Additional file 1 of A cell engineering approach to enzyme-based fed-batch fermentation

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posted on 2021-07-25, 03:21 authored by Michael Sibley, John M. Ward
Additional file 1: Figure S1. Alignment of the glucoamylases from Thermoanaerobacter tengcongensis MB4, Deinococcus geothermalis and Chromobacterium violaecum. Figure S2. Crude (whole cell) and clarified lysates analysed on SDS page for pQR1706, pQR1707 and pQR1708. Figure S3. Growth curves of α-amylase secreting E. coli compared to plasmid free E. coli cultured in commercially available high cell density media. Figure S4. Design of starch-agar layer system for sufficient provision of carbon for high cell density applications.

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Engineering and Physical Sciences Research Council Biotechnology and Biological Sciences Research Council

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    Microbial Cell Factories

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    Enzyme-based fed-batch fermentationBacterial glucoamylaseStarch to glucose conversionCell engineering for bioprocess

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