Additional file 1 of 2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

Additional file 1: Fig. S1. Simulation of nanopore DRS read alignments. Fig. S2. Annotation-guided alignment improves spliced alignment of simulated reads. Fig. S3. Junction metrics can identify genuine splice junctions. Fig. S4. Machine-learned sequence information can identify genuine splice junctions. Fig. S5. Filtered two-pass alignment improves the identification and quantification of correct transcripts without a reference annotation. Fig. S6. Filtered two-pass alignment improves genome-guided annotation.