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Additional file 1: Table S1. of In vivo characterization of an Hfq protein encoded by the Bacillus anthracis virulence plasmid pXO1
journal contribution
posted on 2017-03-14, 05:00 authored by Andrea Keefer, Eugenia Asare, Andrei Pomerantsev, Mahtab Moayeri, Craig Martens, Stephen Porcella, Susan Gottesman, Stephen Leppla, Catherine Vrentasβ-galactosidase assay result summary. Results from two independent trials of the β-galactosidase assay depicted in Fig. 5b. (Each trial involved triplicate independent transformants for each mutant). In these assays, Miller units for each sample were corrected by first subtracting the value for the Hfq1 strain (used as a negative control), and then were compared to the corrected value for the wild-type Hfq3 strain, set to 100%. Mutants with negative values exhibited lower expression than the Hfq1 control strain. While we observed some variability in the signal as a percentage of the wild-type Hfq3 signal between trials, the same rank order of mutant expression levels was observed in each trial. Table S2. Plasmids created in this study. Table S3. Bacterial strains utilized and/or created in this study. Table S4. Primers utilized in this study. Table S5. MIC (μg/mL) of cell wall-targeting antibiotics for Hfq3 and GFP overexpression strains. (PDF 283 kb)