Additional file 1: Figure S1. of Phylogenomic and functional analyses of salmon lice aquaporins uncover the molecular diversity of the superfamily in Arthropoda

Extracellular views of cartoon renders of salmon louse aquaporins illustrating the ar/R constriction residues (spacefill) that determine the molecular selectivity of the channel. Labels are annotated as for Fig. 1 in the main text. Figure S2. Bayesian majority-rule mid-point rooted tree of an amino acid alignment of expressed transcripts in Maxillipoda. The tree was inferred from 500,000 MCMC generations on 8,085 amino acid sites. Posterior probabilities are shown at each node. Scale bar represents the number of amino acid substitutions per site. Grey bars highlight species with N-terminal splice-variants. Figure S3. Bayesian majority-rule mid-point rooted tree of a codon alignment of the aquaporin superfamilies in the Atlantic salmon and salmon louse. The tree was inferred from 1 million MCMC generations on 57,075 nucleotide sites. Posterior probabilities of the codon/amino acid analyses are shown at each node. Scale bar represents the number of nucleotide substitutions per site. Figure S4. In the absence of cAMP, salmon louse Glp1_v1 is non functional in X. laevis oocytes. (A) Osmotic water permeability (P f) of Glp1_v1 without the addition of cAMP compared to water- and glp_3v2-injected oocytes as negative and positve controls, respectively. (B) Immunofluorescence micrographs of paraffin sections of water- (Ctrl) and glp1_v1-injected oocytes in the absence of cAMP probed with paralog-specific antisera followed by Cy3-labeled anti-rabbit IgG. Arrows point to the plasma membrane. Inset: Magnified region shows the retention of Glp1_v1 (green) just below the oocyte plasma membrane. Table S1. C-terminal peptides used to immunize rabbits to raise salmon louse affinity-purified antibodies. Table S2. List of aquaporin accession numbers used in the study. Table S3. Oligonucleotide primers used for RT-PCR analysis. Nucleic acid sequences for primers specific for each salmon louse aquaporin mRNA, ef1α and expected product size. (PDF 2063 kb)